Silaphet Somphavong
Center for Infectiology Lao-Christophe Merieux, Lao PDR
Title: First insights into the genetic characteristics and drug resistance of Mycobacterium tuberculosis population collected during the first National Tuberculosis Prevalence Survey of Lao PDR (2010–2011)
Biography
Biography: Silaphet Somphavong
Abstract
In Lao PDR, little is known about the genetic characteristics and drug resistance (DR) of the Mycobacterium tuberculosis (M.tb). We aimed to study the genetic characteristics and DR of M. tb collected during the first National TB Prevalence Survey (2010–2011) in order to better understand the TB epidemiology in the country. Using MTBDRplus test for DR detection and spoligotyping and MIRU-VNTR for genotyping.
Of 222 M. tb isolates, 11 were INH mono-resistant, 2 were MDR-TB. Of 206, the proportion of EAI family was 75.7%, Beijing 14.4% and T 5.5%. EAI isolates came from all provinces, Beijing isolates were found mainly in the northern provinces. A proportion of Beijing isolates was higher in people younger than 35 years compared to EAI, the percentage of DR was higher among Beijing (17.2%) than EAI (5.2%) isolates, the two MDR-TB isolates belonged to the Beijing family. The MIRU-VNTR and spoligotyping results revealed an estimated clustering rate of 11% and the occurrence of mini-outbreaks of DR-TB caused by Beijing genotypes.